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semi dry western blot apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad semi dry western blot apparatus
    Semi Dry Western Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 3013 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semi+dry+western+blot+apparatus/Mini-PROTEAN+Tetra+Cell/pmc11049330-80-1-8
    Average 97 stars, based on 3013 article reviews
    semi dry western blot apparatus - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Monoclonal IgA Antibodies for Aflatoxin Immunoassays
    Article Snippet: .. For Western blot analysis, the proteins on the gels were transferred on a PVDF membrane in methanol containing transfer buffer (0.02 M Trizma base, 0.15 M Glycin, 0.0003% SDS and 20% Methanol) using a semi‐dry Western blot apparatus (Bio‐ Rad, Hercules, CA, USA, Trans‐Blot Turbo Transfer System) for 30 min at 200 mA. ..

    Article Title: Changes in AMPKα and Ubiquitin Ligases in Myocyte Reverse Remodeling after Surgical Ventricular Reconstruction in rats with ischemic cardiomyopathy.
    Article Snippet: .. A semi-dry western blot apparatus (Mini-PROTEAN Tetra Cell, BIO-RAD, CA, USA) was used for western blotting. .. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% Mini-PROTEANTGXTM, BIO-RAD, CA, USA), the proteins were blotted onto polyvinylidene difluoride membranes and incubated with primary antibodies (AKT, p-AKT [Ser473], AMPKα, p-AMPKα [Thr172], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], p62/SQSTM1, ubiquitin: Cell Signaling, MA, USA; antiLC3B, Abcam, Cambridge, UK) and secondary antibodies (anti-rabbit IgG, Cell Signaling).

    Article Title: Effects of Trehalose Preconditioning on H9C2 Cell Viability and Autophagy Activation in a Model of Donation after Circulatory Death for Heart Transplantation
    Article Snippet: After adding lysis buffer (FUJIFILM Wako Pure Chemical Corporation) containing 1% proteinase inhibitor (FUJIFILM Wako Pure Chemical Corporation), the total protein expression was assessed using the Bradford method. .. A semi-dry Western blot apparatus (Mini-PROTEAN Tetra Cell; Bio-Rad) was used to detect the conversion from LC3-I (cytosolic form) to LC3-II (membrane-bound lipidated form). .. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% Mini-PROTEANTGX; Bio-Rad), proteins were blotted onto polyvinylidene difluoride membranes and incubated with primary (Anti-LC3B; 1:500, Abcam, Cambridge, UK) and secondary antibodies (Anti-rabbit IgG; 1:2000, Cell Signaling Technology, Danvers, MA, USA).

    Membrane:

    Article Title: Monoclonal IgA Antibodies for Aflatoxin Immunoassays
    Article Snippet: .. For Western blot analysis, the proteins on the gels were transferred on a PVDF membrane in methanol containing transfer buffer (0.02 M Trizma base, 0.15 M Glycin, 0.0003% SDS and 20% Methanol) using a semi‐dry Western blot apparatus (Bio‐ Rad, Hercules, CA, USA, Trans‐Blot Turbo Transfer System) for 30 min at 200 mA. ..

    Article Title: Effects of Trehalose Preconditioning on H9C2 Cell Viability and Autophagy Activation in a Model of Donation after Circulatory Death for Heart Transplantation
    Article Snippet: After adding lysis buffer (FUJIFILM Wako Pure Chemical Corporation) containing 1% proteinase inhibitor (FUJIFILM Wako Pure Chemical Corporation), the total protein expression was assessed using the Bradford method. .. A semi-dry Western blot apparatus (Mini-PROTEAN Tetra Cell; Bio-Rad) was used to detect the conversion from LC3-I (cytosolic form) to LC3-II (membrane-bound lipidated form). .. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% Mini-PROTEANTGX; Bio-Rad), proteins were blotted onto polyvinylidene difluoride membranes and incubated with primary (Anti-LC3B; 1:500, Abcam, Cambridge, UK) and secondary antibodies (Anti-rabbit IgG; 1:2000, Cell Signaling Technology, Danvers, MA, USA).



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